Protocols

 

  • Anthracnose Leaf Blight Assay
  • Basic PCR
  • Cultures preparartion for RNA extraction
  • Detection of differentially expressed genes using RNA-Seq and CyVerse
  • Digestion of DNA with restriction endonucleases
  • DNA cleaning RT-PCR
  • Electroporation Protocol
  • Fungal cultures preparation for maxi DNA extraction
  • Fungal Protoplast Preparation
  • Genomic DNA Extraction From Fungi (maxi_DNA extraction)
  • How to make Solutions
  • Media
  • Nucleic acid concentration and/or cleaning
  • Pathogen Identification using DNA Barcoding
  • Plasmid Rescue
  • Primer Preparation
  • Recipes
  • Recovery of C. graminicola from Root Infected Plants
  • Root Inoculation Assay
  • Seed sterilization
  • Seed sterilization (of corn seeds for root inoculations)
  • SOP
  • Southern Blots
  • Spore germination assay
  • Storage of Fungi on Silica Gel
  • Transformation of C. graminicola to Hygromycin resistance
  • Working with RNA

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