1. Apply fungicide.
Flint 50WG (Trifloxysfrobin) 0.8g fungicide/ Kg of seed
0.008g fungicide/ 10g seed in 0.5mL ddH2O
2. Allow fungicide to dry over night.
3. Rinse seeds with water.
4. Surface sterilize seeds by washing in a 70% ethanol solution for 5 min.
5. Wash the seeds in a 10% hydrogen peroxide solution for 10 min.
6. Rinse the seeds with water three times.