Primers are delivered as dry pellets and can be storage at 4C.
-Use sterile 1.5ml centrifuge tubes, add to each primer tube received 1ml nuclease free water using filter tips.
-Adjust the concentration of your primers to a final of 10mMolar. For example if primer conc. is 32.8 nanomol (nmol) its CM= (32.8 10-9Mole) /1*10-3 L = 32.8 uM
-if I want to make 500 ul of 10uM, how many ul of primers should I use?
Vi= initial volume
Ci= initial concentration = 32.8 uM
Vf= final volume = 500 ul
Cf= final concentration = 10 uM
Vi x Ci = Vf x Cf then
Vi= Vf x Cf/ Ci =
Vi= 500 ul x10uM / 32.8 uM
Vi= 153 ul
153 ul of original primer to make 500 ul of 10uM primer, then
153 ul of primer and 347 ul of water for a final concentration of 10 uM
store at –20°C