Seed sterilization

(from Slavica)


5 minutes 70% ethanol.

1:45 hours 10% H2O2


In a 50 mL tube, rinse seeds with sterile water 4-5 times.


If seeds will be used for In vitro tissue culture:

Seeds will float on H2O2, so pass through a sterile funnel covered with filter paper.

Dry on a sterile filter paper and germinate on filter paper in a Petri dish for 2 days (by adding 5 mL of water to the paper).

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