Basic PCR, 25 ul
Use tips and tubes nuclease free
VOL
10xPCR buffer * 2.5 ul (1x)
10mM dNTPmix 0.5 ul (200uM)
50mM MgCl2 0.8 ul (1.6 mM)
Primer Mix 10uM 1.5 ul each (0.6 uM)
Template (100-150ng) 2 ul
Taq 5U/ul 0.5 ul
Nuclease free water 15.7 ul
Termocycler Program
Lid 110 C
Preheat off
For 35 cycles
94 C, 1 min
Denature 94 C 1 minutes
Annel xx C 40 sec
Exten 72 C 1minute
10 min at 72C
*(The some brand than the taq)